recoder scans a given nucleotide sequence for restriction sites. It reports single base positions in the restriction pattern which when mutated remove the restriction site while maintaining the same translation (in frame 1 of the input sequence).

Here is a sample session with recoder:

% recoder Find and remove restriction sites but maintain the same translation Input sequence: em:hsfau Comma separated enzyme list [all]: EcoRII Output file [hsfau.recoder]:

Mandatory qualifiers:

[-seq] (sequence)

Nucleic acid sequence.

-enzymes (string)

Comma-separated enzyme list

[-outf] (outfile)

Results filename

Advanced qualifiers:

-sshow (boolean)

Display untranslated sequence.

-tshow (boolean)

Display translated sequence.

Sequence Analysis in a Nutshell
Sequence Analysis in a Nutshell: A Guide to Common Tools and Databases
ISBN: 059600494X
EAN: 2147483647
Year: 2005
Pages: 312

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