dan calculates the melting temperature (Tm) and the percent G+C of a nucleic acid sequence (optionally plotting them). Here is a sample session with dan: % dan Input sequence: embl:paamir Enter window size [20]: Enter Shift Increment [1]: Enter DNA concentration (nM) [50.]: Enter salt concentration (mM) [50.]: Output file [paamir.dan]: An example of producing a plot of Tm: % dan -plot Input sequence(s): embl:paamir Enter window size [20]: Enter Shift Increment [1]: Enter DNA concentration (nM) [50.]: Enter salt concentration (mM) [50.]: Enter minimum temperature [55.]: Graph type [x11]: Mandatory qualifiers (bold if not always prompted):
Optional qualifiers (bold if not always prompted):
Advanced qualifiers:
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