recoder scans a given nucleotide sequence for restriction sites. It reports single base positions in the restriction pattern which when mutated remove the restriction site while maintaining the same translation (in frame 1 of the input sequence). Here is a sample session with recoder: % recoder Find and remove restriction sites but maintain the same translation Input sequence: em:hsfau Comma separated enzyme list [all]: EcoRII Output file [hsfau.recoder]: Mandatory qualifiers:
Advanced qualifiers:
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