msbar changes a sequence a lot or a little, attempting to emulate various forms of mutation. You can set the number and types of mutations. Here is a sample session with msbar. This asks for 5 mutations, with point mutations as changes (substitutions) and the codon and block mutations ignored: % msbar Input sequence: embl:eclaci Output sequence [eclaci.fasta]: Number of times to perform the mutation operations [1]: 5 Point mutation operations 0 : None 1 : Any of the following 2 : Insertions 3 : Deletions 4 : Changes 5 : Duplications 6 : Moves Types of point mutations to perform [0]: 4 Codon mutation operations 0 : None 1 : Any of the following 2 : Insertions 3 : Deletions 4 : Changes 5 : Duplications 6 : Moves Types of codon mutations to perform [0]: Block mutation operations 0 : None 1 : Any of the following 2 : Insertions 3 : Deletions 4 : Changes 5 : Duplications 6 : Moves Types of block mutations to perform [0]: Mandatory qualifiers (bold if not always prompted):
Optional qualifiers (bold if not always prompted):
Advanced qualifiers:
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